|Safia Omer, 4th Year
Advisor: Wei-Lih Lee
Education:University of Khartoum, Institute of Endemic Diseases, Khartoum, Sudan
M.Sc. Molecular Medicine, 2010
Thesis title: Copy Number Variation of Plasmodium falciparum Reticulocyte Binding Protein Homologue 4 (PfRh4)
University of Khartoum, Khartoum, Sudan, B.Sc. with Honors, Zoology 2007
Steven Markus*, Safia Omer*, Kaitlyn Baranowski, Wei-Lih Lee. Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae. 2015. Traffic Jul;16(7):773-86. doi: 10.1111/tra.12276.
* Co-First Authorship
Research spindle dynamics during mitosis in budding yeast
My studies aim to understand how dynein activity is regulated at the cortex in budding yeast. In the past year, I have generated data that support the role of cortical attachment molecule, Num1 in regulating dynein activity. I showed, via utilizing Num1 localization mutants, which altered Num1 localization uncovers new dynein-dependent cortical pulling mechanism that is negatively regulated in wild-type cells. I further showed that this mechanism requires dynein interaction with Num1 at the cortex and its motor activity. Additionally, I identified that Num1 distribution along the cortex is able to regulate and facilitate dynein switching between two modes of cortical pulling mechanisms – microtubule sliding and end-on capture-shrinkage. These data are part of a manuscript in preparation.